* You are viewing Posts Tagged ‘Gel’

The dangers of overexposing western blots

Nigel
It’s like, how much more black could this be? And the answer is none. None more black.

Nigel Tufnel, lead guitarist of Spinal Tap.

 

None more black (or none more white) is a real concern when you’re talking about imaging western blots. If you only want to verify the presence of a protein, you can stop reading here, because even an overexposed image will tell you that there is some protein present in your sample. But an overexposed image of a western blot is essentially useless when you’re … Continue Reading

Analyzing gels and western blots with ImageJ

The following information is an updated version of a method for using ImageJ to analyze western blots from a now-deprecated older page.

If you’re looking for a more comprehensive workflow option for your western blot analyses, please visit my tutorial on using Image Studio LiteIS_lite_icon, a free software package from LI-COR Biosciences. The Image Studio Lite software can be downloaded for free from www.licor.com/islite. In addition to the functionality described below for ImageJ, Image Studio Lite provides additional … Continue Reading