The littorine snail in that kid’s knee

This news story about a seashore snail found living in a child’s knee wound has been making the rounds lately.

Apparently 4-year old Paul Franklin from Southern California was camping at a beach in central California near Morro Bay, when he fell and hurt his knee (Orange County Register). For several weeks afterwards, the wound refused to heal fully, despite the use of … Continue Reading

The dangers of overexposing western blots

Nigel
It’s like, how much more black could this be? And the answer is none. None more black.

Nigel Tufnel, lead guitarist of Spinal Tap.

 

None more black (or none more white) is a real concern when you’re talking about imaging western blots. If you only want to verify the presence of a protein, you can stop reading here, because even an overexposed image will tell you that there is some protein present in your sample. But an overexposed image of a western blot is essentially useless when you’re … Continue Reading

Stupid water bath tricks

As part of the downward spiral into increasingly esoteric subjects that is currently happening on this site, I now present some info on making a water bath do things. Simple things, but things nonetheless.

An old Cole-Parmer Digital Polystat water bath.

An old Cole-Parmer Digital Polystat water bath.

 

As part of an ongoing project in the lab, it became necessary to start carrying out simple temperature ramps with a water bath. For instance, we’ll start from a temperature of 15°C and go to a target temperature of 35°C at a … Continue Reading

O2 conversion

The molar volume of an ideal gas is ~22.4 liters per mole (we’ll  consider O2 to be an ideal gas for these purposes, and 22.4 is an approximation that varies with temperature, it’s 22.414 at 0C). Convert from 22.4 liters to milliliters ( = 22400 ml per mol). So if you have 1 milliliter of an ideal gas, there is 1 ml / 22400 ml per mol = 0.00004464 mol of that gas, which is 44.64 µmol of the gas. Thus, if you have a volume of a gas in ml, you can calculate the µmols of that gas simply … Continue Reading

Removing security features from a pdf

Whoops, I totally made my own pdf and accidentally included a bunch of security features, so now I can’t use my highlighter or type notes on the document. It sure would be handy to take off those security features that I put there myself when I made the pdf, myself.

With a full copy of Adobe Acrobat and the associated Acrobat Distiller software, the process is as follows:

1. Open the secured pdf in Acrobat.

2. Go to File>Export>Postscript>Postscript and save a .ps version of the pdf file.

3. Open the .ps postscript file in a text editor (WordPad on Windows works fine).

4. Use … Continue Reading

More tide prediction with R

Edit: There is now a full-fledged R package, rtide, to accomplish the same basic task of generating time series of tide predictions that is outlined here. See this more recent post for information.

In the previous post I outlined how to query the XTide software with R and parse the results into a handy-dandy data frame. The biggest hurdle with that method is getting XTide up and running on your computer. The code outlined here works entirely within R, so you don’t need XTide installed on your computer. The trade-off is that it provides substantially … Continue Reading

Interfacing XTide and R

Edit: There is now a full-fledged R package, rtide, to accomplish the same basic task of generating time series of tide predictions that is outlined here. See this more recent post for information.

XTide is an open-source program that predicts tide heights and current speeds for hundreds of tide and current stations around the United States. It can be used to produce tide predictions in the past and future for a site at your chosen interval (down to the minute), as well as producing sunrise and sunset times, moon phases, and times when the tide level … Continue Reading

Book Review: The R Book, Second Edition (2013)

The first edition of The R Book by Michael J. Crawley was an ambitious work, but managed to be slightly rubbish due to the atrocious typographical layout of the original book. The good news is that the new 2nd edition, released in 2013, has a substantially improved layout that makes the book far more useful as a general reference. This is important, since the book is meant as an accessible reference for non-statisticians to many of the powerful data manipulation and statistical techniques available in R, particularly for biologists and researchers in similar fields. With this new edition you’re no … Continue Reading

ImageJ StartupMacros

Before I lose this file again: StartupMacros.txt This is a text file, based on the original StartupMacros.txt file that came with ImageJ, that can be dropped in the ImageJ/macros folder that is created when you install ImageJ. Start up ImageJ, and if this macro doesn’t automatically run, go to Plugins>Macros>Startup Macros to load it. It installs a few more drawing tools on the ImageJ toolbar and creates some keyboard shortcuts for the following tools:

  • Line tool – press ‘l’
  • Brush tool – press ‘b’
  • Eraser tool – press ‘e’
  • Wand tool – press ‘w’

I either found or created some code … Continue Reading

Analyzing western blots with Image Studio Lite

IS_lite_iconImage Studio Lite is a free software package from LI-COR Biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. Although the Lite version is free, there is a more comprehensive paid version of the software that aims to easily integrate with the apparatus that Licor also sells. However, the free Image Studio Lite is more than sufficient for basic analysis of western blots, as I’ll show below.

Obviously you need to start by downloading and installing Image Studio Lite. The first time … Continue Reading

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