Category: Western blotting
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The dangers of overexposing western blots
It’s like, how much more black could this be? And the answer is none. None more black. – Nigel Tufnel, lead guitarist of Spinal Tap. None more black (or none more white) is a real concern when you’re talking about imaging western blots. If you only want to verify the presence of a protein,…
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Analyzing western blots with Image Studio Lite
Image Studio Lite is a free software package from LI-COR Biosciences aimed at life scientists that want to analyze gels, western blots, dot blots, and other similar lab outputs. Although the Lite version is free, there is a more comprehensive paid version of the software that aims to easily integrate with the apparatus that Licor…
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Analyzing gels and western blots with ImageJ
How to use the free program Image-J to quantify bands on gels and western blots.