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Analyzing gels and western blots with ImageJ

The following information is an updated version of a method for using ImageJ to analyze western blots from a now-deprecated older page. If you need a peer-reviewed citation for the methods outlined below, you may cite  Stael, S., L.P. Miller, Á.D. Fernández-Fernández and F. Van Breusegem (2022). Detection of Damage-Activated Metacaspase Activity by Western Blot in Plants. Plant Proteases and Plant Cell Death: Methods and Protocols. M. Klemencic, S. Stael and P.F. Huesgen. New York, NY, Springer US: 127-137 https://doi.org/10.1007/978-1-0716-2079-3_11

If you’re looking for a more comprehensive workflow option for your western blot … Continue Reading