Tag: ImageJ
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The dangers of overexposing western blots
It’s like, how much more black could this be? And the answer is none. None more black. – Nigel Tufnel, lead guitarist of Spinal Tap. None more black (or none more white) is a real concern when you’re talking about imaging western blots. If you only want to verify the presence of a protein,…
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ImageJ StartupMacros
Before I lose this file again: StartupMacros.txt This is a text file, based on the original StartupMacros.txt file that came with ImageJ, that can be dropped in the ImageJ/macros folder that is created when you install ImageJ. Start up ImageJ, and if this macro doesn’t automatically run, go to Plugins>Macros>Startup Macros to load it. It…
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Digitizing data from old figures with ImageJ
Following on this earlier post concerning the retrieval of data from figures in published papers with R, here is a method for doing the same with ImageJ. This is a freely available program that should run on Windows/Mac/Linux, just like R. To extract data points from a figure, you’ll need the Figure_Calibration plugin developed by…
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ImageJ paint brush tool keyboard shortcut
ImageJ is easy to modify if you know a bit of Java. I don’t know any Java. But here’s what I did to add keyboard shortcuts for the paint brush tool and the line selection tool. The code is based off this help file from the ImageJ website: http://rsbweb.nih.gov/ij/docs/guide/userguide-32.html The first step was to figure…
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Measuring leaf or algae blade area in a digital photo with ImageJ
The following is a tutorial on using the freely available ImageJ program from the National Institutes of Health for estimating the area of a leaf or algae blade (or anything else). The process is straightforward once you understand the various steps. You must begin with a photograph of your object, flattened and dried off as…
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Analyzing gels and western blots with ImageJ
How to use the free program Image-J to quantify bands on gels and western blots.