The dangers of overexposing western blots
Tags: Gel, Image Studio Lite, ImageJ, Spinal Tap, Western blot
It’s like, how much more black could this be? And the answer is none. None more black.
– Nigel Tufnel, lead guitarist of Spinal Tap.
None more black (or none more white) is a real concern when you’re talking about imaging western blots. If you only want to verify the presence of a protein, you can stop reading here, because even an overexposed image will tell you that there is some protein present in your sample. But an overexposed image of a western blot is essentially useless when you’re … Continue Reading